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KMID : 1094719990040030181
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Biotechnology and Bioprocess Engineering
1999 Volume.4 No. 3 p.181 ~ p.181
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Effect of dissolved oxygen concentration onnar promoter activity in batch and semi-continuous cultivations
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Hong Eock-Kee
Kim Kwang-Soo
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Abstract
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The level of the dissolved oxygen concentration could significantly affect thenar promoter activity in the induction of the gene expression under the anaerobic condition. In batch culture, the β-galactosidase activity was about 12000 units/min/g cell under the anaerobic condition. The optimum DO concentration for the induction of the gene expression was 0% in both batch and continuous cultivations. In semi-continuous culture, the maximum enzyme activity was about 11000 units/min/g cell at 0% of the DO concentration. These results indicated that the absolutely anaerobic culture condition was required for the maximum gene expression.
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KEYWORD
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E. coli, nar promoter, DO concentration, anaerobic condition
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